Our client Alexander is a Biological Technician with 10 years of experience in the biotechnology industry. Skilled in Fermentation Process Development, SDS-PAGE, Molecular Biology, Protein Folding, and Cell Culture, in a process development environment.
About Alexander:
Biological Technician with 10 years of experience in the biotechnology industry. Skilled in Fermentation Process Development, SDS-PAGE, Molecular Biology, Protein Folding, and Cell Culture, in a process development environment. Supportive team member with an easy-going personality and clear and open communication skills (fluent in English and German). Experienced in training and mentoring new staff and skilled in using programs such as MS-Office, FlowJo and Genious
Spectrum of scientific methods
- Production of FAB fragments
- The spectrum of molecular cloning methods for the development of FAB fragments
- Expression and purification of FAB fragments in E.coli systems
- Generation and screening of bacterial glycerol stocks
- Protein purification using an AKTA Pure 25 system
- Establishment and maintenance of a Multifor fermenter (4x1L)
- Design and production of diverse culture media for growth- and expression optimization
- Process development and scaling for SAM2-transfer
Biomolecular methods
- Molecular cloning, protein expression
- Primer design
- DNA extraction and purification
- Cloning: restriction based, 3-way –Gateway reactions, Gibson-Assambley
- Immunoblotting, immunocytochemistry
- SDS-Page, Coomassie Staining
- PCR, long-range PCR
- DNA / RNA isolation and quantitation
- ELISA, various absorption- und fluorescence-based assays
Cell culture
- Murine embryonic stem cells
- Cell lines and transfection cells: lipofection
