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Candidate Details
Name :
Alexander Agapov
Qualification :
Laboratory Technician
Industry :
Life Sciences - Vaccine Generation Research
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Candidate ID:
AA31
This candidate has been hired.

Laboratory Technician

About our Client

Biological Technician with 10 years of experience in the biotechnology industry. Skilled in Fermentation Process Development, SDS-PAGE, Molecular Biology, Protein Folding, and Cell Culture, in a process development environment. Supportive team member with an easy-going personality and clear and open communication skills (fluent in English and German). Experienced in training and mentoring new staff and skilled in using programs such as MS-Office, FlowJo and Genius.

Achievements

Professional Experience

Education

Interests

Skills

Technical Skills

Spectrum of scientific methods

  • Production of FAB fragments
  • The spectrum of molecular cloning methods for the development of FAB fragments
  • Expression and purification of FAB fragments in E.coli systems
  • Generation and screening of bacterial glycerol stocks
  • Protein purification using an AKTA Pure 25 system
  • Establishment and maintenance of a Multifor fermenter (4x1L)
  • Design and production of diverse culture media for growth- and expression optimization
  • Process development and scaling for SAM2-transfer


Biomolecular methods

  • Molecular cloning, protein expression
  • Primer design
  • DNA extraction and purification
  • Cloning: restriction based, 3-way –Gateway reactions, Gibson-Assambley
  • Immunoblotting, immunocytochemistry
  • SDS-Page, Coomassie Staining
  • PCR, long-range PCR
  • DNA / RNA isolation and quantitation
  • ELISA, various absorption- und fluorescence-based assays


Cell culture

  • Murine embryonic stem cells
  • Cell lines and transfection cells: lipofection

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